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Genotyping and Gene Expression Core Facility
The CMMT Genotyping Core Facility provides high-throughput genotyping services, gene expression profiling, and DNA purification services to researchers in the academic, industrial, and pharmaceutical communities.
Genotyping Services
Genotyping is used for identifying candidate gene regions for genetic studies via genome-wide association studies, genome-wide linkage studies plus fine mapping panels, and custom candidate gene approaches assaying 96-1536 Single Nucleotype Polymorphisms (SNPs) at one time.
The Facility offers a range of products and services for high-confidence, cost-effective genotyping using the Illumina gentyping platform. It offers whole-genome content, focused content, and broad flexibility for custom SNP content. Content is deployed on one of two bead-based array platforms and supported by two powerful genotyping protocols: the Infinium™ Assay and the GoldenGate® Assay.
The key advantage of Illumina’s technology is the array of beads. Each bead type is assigned to a specific SNP locus and replicated 30 times on each array, meaning that each genotype call is the result of the mean intensity of all 30 replicates, resulting in industry-leading accuracy (>99.9%), reproducibility (99.99%), call rates (>99.7%), and low cost.
Gene Expression Profiling Services
The CMMT’s gene expression profiling uses Illumina technology to deliver the latest high-performance arrays. Illumina arrays interrogate multiple whole genomes or focused sets of target genes across large sample populations with very low cost per sample.
The key advantage of Illumina’s technology for RNA expression analysis is the array of beads. The 30-fold feature redundancy (30 average replicates for each transcript) delivers industry-leading high-confidence results. The high feature density reduces array-to-array variation, making single sample replicates less important than more meaningful biological sample replicates. The high feature density also enables low sample and reagent volumes, resulting in low per-sample pricing and the ability to make larger experiments feasible.
For more information: www.cmmt.ubc.ca/genotyping
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